Adding dried distillers grains with solubles influences the rumen microbiome of meat goats fed lespedeza or alfalfa-based diets

We evaluated the effect of replacement corn and soybean meal (SBM) with dried distillers grains solubles (DDGS) in sericea lespedeza (SL; Lespedeza cuneata) alfalfa (Medicago sativa)-based diets on ruminal fermentation bacterial community growing meat goats. Thirty-six male Spanish goats an average body weight 24 ± 1.8 kg were stratified by assigned to four isonitrogenous (crude protein = 17.1 0.1%) isocaloric (3.2 0.09 Mcal/ DM) for 60 d a randomized design 2 × factorial arrangement. The dietary treatments (1) alfalfa-based diet (ALC), (2) + DDGS (ALD; SBM DDGS), (3) SL-based (SLC) (4) SL (SLD; DDGS). slaughtered, rumen contents immediately sampled 16 S rRNA gene sequencing analysis. analysis Similarity (ANOSIM) linear discriminant (LDA) size (LEfSe) respectively used detect microbial shift differentially abundant taxa among treatments. Addition increased (P < 0.05) acetate concentrations both lespedeza-based diets. However, reduced butyrate ammonia nitrogen concentrations, compared ANOSIM results revealed between ALC ALD (R 0.251; P 0.01) SLC SLD 0.289; 0.001). LEfSe that relative abundance 7 taxa, including Ruminococcaceae UCG 002, Succinivibrionaceae Prevotellaceae YAB2003, Lachnospiraceae 010 (LDA > 2; while 38 enriched ALC. Similarly, 31 Ruminococcus YE281, Succinivibrio, ND3007 reduced, 35 SLC. In conclusion, this study showed regardless forage type, inclusion modulates population fibrolytic butyrate-producing bacteria